近日，華中農(nóng)業(yè)大學(xué)植物科學(xué)技術(shù)學(xué)院農(nóng)藥毒理學(xué)及有害生物抗藥性團(tuán)隊(duì)研究成果以“Microbiome variation correlates with the insecticide susceptibility in different geographic strains of a significant agricultural pest, Nilaparvata lugens ”為題在npj Biofilms and Microbiomes發(fā)表。

 

　　研究提出了“害蟲抗藥性微生物組”的概念，揭示了不同褐飛虱田間種群中微生物豐度及多樣性與殺蟲劑敏感性的聯(lián)系，將害蟲微生物組納入害蟲抗藥性監(jiān)測(cè)的范疇，鑒定了褐飛虱抗藥性的重要微生物標(biāo)記，研究結(jié)果深化了害蟲抗藥性的認(rèn)識(shí)，亦有望為“靶菌治蟲”策略的實(shí)施、害蟲抗藥性治理及新藥創(chuàng)制提供新的策略和靶標(biāo)。

 

　　研究團(tuán)隊(duì)立足我國水稻綠色高質(zhì)量安全生產(chǎn)的重大需求，緊緊圍繞水稻重要害蟲褐飛虱的抗藥性問題開展系列研究。連續(xù)十年系統(tǒng)地開展褐飛虱抗藥性監(jiān)測(cè)，積累了寶貴的抗性基礎(chǔ)數(shù)據(jù)與抗性資源；前期研究揭示了褐飛虱的共生細(xì)菌Wolbachia調(diào)控褐飛虱解毒代謝的分子機(jī)制（Zhang et al., 2021）。

 

　　然而，昆蟲微生物組多樣性如何在復(fù)雜田間環(huán)境下塑造褐飛虱殺蟲劑抗藥性尚不清楚。基于此，本研究通過廣泛比較多個(gè)褐飛虱田間及實(shí)驗(yàn)室種群的微生物組組成、轉(zhuǎn)錄本表達(dá)水平和對(duì)不同殺蟲劑的敏感性，提出了“抗藥性微生物組”的概念，通過聯(lián)合分析發(fā)現(xiàn)，褐飛虱核心細(xì)菌共生體的豐度與宿主抗藥性相關(guān)解毒基因的表達(dá)顯著相關(guān)，明確非生物環(huán)境因子驅(qū)動(dòng)的褐飛虱田間種群微生物組多樣性塑造了宿主解毒酶基因表達(dá)模式進(jìn)而導(dǎo)致不同田間種群殺蟲劑抗性變異。

 

　　該研究鑒定了多種褐飛虱核心共生菌作為其抗性水平的“Biomarker”，為今后田間抗性水平快速監(jiān)測(cè)技術(shù)的開發(fā)提供了有力支撐，同時(shí)為氣候變化背景下田間害蟲對(duì)殺蟲劑抗性的發(fā)展及治理策略制定提供了新視角。

 

　　植物科學(xué)技術(shù)學(xué)院已畢業(yè)博士張?jiān)乞懞筒┦垦芯可掏⑿l(wèi)為該論文共同第一作者，萬虎教授為論文通訊作者，李建洪教授、何順副教授和佛羅里達(dá)大學(xué)Adam C. N. Wong助理教授參與了研究的指導(dǎo)，以上研究得到國家自然科學(xué)基金、國家重點(diǎn)研發(fā)計(jì)劃等項(xiàng)目的資助。

 

　　【英文摘要】

 

　　Microbiome-mediated insecticide resistance is an emerging phenomenon found in insect pests. However, microbiome composition can vary by host genotype and environmental factors, but how these variations may be associated with insecticide resistance phenotype remains unclear. In this study, we compared different field and laboratory strains of the brown planthopper Nilaparvata lugens in their microbiome composition, transcriptome, and insecticide resistance profiles to identify possible patterns of correlation. Our analysis reveals that the abundances of core bacterial symbionts are significantly correlated with the expression of several host detoxifying genes （especially NlCYP6ER1, a key gene previously shown involved in insecticides resistance）。 The expression levels of these detoxifying genes correlated with N. lugens insecticide susceptibility. Furthermore, we have identified several environmental abiotic factors, including temperature, precipitation, latitude, and longitude, as potential predictors of symbiont abundances associated with expression of key detoxifying genes, and correlated with insecticide susceptibility levels of N. lugens. These findings provide new insights into how microbiome-environment-host interactions may influence insecticide susceptibility, which will be helpful in guiding targeted microbial-based strategies for insecticide resistance management in the field.

 

　　論文鏈接：https://www.nature.com/articles/s41522-023-00369-5